Direct and Derivative Spectrophotometric Determination of Copper (II) using 3, 5-Dimethoxy-4-hydroxy Benzaldehyde Isonicotinoyl Hydrazone (DMHBIH)

Abstract

Derivative spectrophotometric determination of Copper (II) with 3, 5 – Dimethoxy – 4 – hydroxyl benzaldehyde isonicotinoylhydrazone (DMHBIH) reagent has been proposed. Direct and derivative method has been developed for the spectrophotometric determination of
micro amounts of Copper (II) in basic buffer solution(pH 9.0). The reagent DMHBIH gives bright yellow coloured water soluble complex
(Cu(II)- DMHBIH  in basic buffer solution. The maximum absorbance was observed in the pH range 8.0-9.5. The molar absorptivity and sandell’s sensitivity of Copper (II) complex with DMHBIH at  max 440 nm was found to be 3.37 x 104 L.mol-1cm-1 and 0.00296 µg/cm2. Beer’s law validity range varies from 0.317 to 3.17 µg /ml. Copper (II) forms 1:1 complex with DMHBIH and stability constant of Copper (II) complex was 21.3X106. The first order derivative amplitude was measured by the peak height method at  max 494 nm. The second order derivative amplitude was measured by the peak height method at  max 510 nm. The developed spectrophotometric method was applied for the determination of Copper (II) in Beer, wine, vegetables and milk samples.